Losartan Potassium and Atenolol are used in combination for treatment of blood pressure. The present work deals with simple spectrophotometric method development for simultaneous estimation of Losartan Potassium (LOS K) and Atenolol (ATE) in two component tablet formulation. The method employed is a first order derivative spectroscopy. For determination of sampling wavelength, 10 μg/ml of each of LOS K and ATE were scanned in 200-400 nm range and sampling wavelengths were 222.4 nm for LOS K where ATE showed zero crossing point and 274 nm for ATE where LOS K showed zero crossing point in first order derivative spectroscopy. For this method linearity observed in the range of 10-30 μg/ml for both ATE and LOS K. The recovery studies confirmed accuracy of proposed method and low values of standard deviation confirmed precision of method. The method is validated as per ICH guidelines.
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